Ku70

Cat.No. 10723-1-AP. KD/KO Validated. KU70. Education . Community See All. 1,579 people like this. 1,603 people follow this.

Ku70

Jun 22, 2010 Ku70 and Ku80 are part of a high‐affinity heterodimeric protein complex, known as Ku (19, 27). The Ku heterodimer binds nonspecifically to DNA Ku70 is a protein that, in humans, is encoded by the XRCC6 gene. Contents. 1 Function; 2 Aging; 3 Clinical; 4 Nomenclature; 5 Interactions; 6 References Jun 1, 2001 Background and Purpose—Ku70 and Ku86, multifunctional DNA repair proteins, bind to broken DNA ends, including double-strand breaks, Anti-Ku70 Protein Antibody from rabbit; Synonym: X-ray repair cross- complementing protein 6, 5′-deoxyribose-5-phosphate lyase Ku70, 5′-dRP lyase Ku70, Heterodimers of the 70 and 80 kDa Ku autoantigens (Ku70 and Ku80) activate the DNA‐dependent protein kinase (DNA‐PK). Mutations in any of the three Ku70 or Ku80 overexpression by transfection with the Ku70 or Ku80 expression gene, respectively, enhanced proliferation of cells with low NF-κB levels.

(B) Ku70 2/ and Ku70 2/ Rap1 D/ (dko) mice display a significant (P,0.001) reduction in testis size compared with wild type (WT) and The DNA end-joining protein Ku70 is one of several proteins that inhibit apoptosis by sequestering the proapoptotic factor Bax from the mitochondria. However, the molecular mechanism underlying Ku70-dependent inhibition of Bax is not fully understood. Here, we show that the absence of Ku70 results in the accumulation of ubiquitylated Bax. Under normal growth conditions, Bax ubiquitylation The protein of 70-kDa molecular weight is a DNA-helicase involved in the DNA double-strand break repair system (Double-Strand Break DNA Repair), more precisely the nonhomologous end joining. Securin 2021-02-22 · Acetylation modulates DNA-mediated Ku70-dependent IFN-λ1 induction.

Plasmid pEGFP-C1-FLAG-Ku70 from Dr. Steve Jackson's lab contains the insert Ku70 and is published in J Cell Biol. 2013 Jul 29. This plasmid is available through Addgene. Se hela listan på academic.oup.com of the Ku70/80 heterodimer in vitro. We further deﬁned the function of the Ku70 and Ku80 C-terminal domains in DNA end binding and DNA-PKCS interaction. 2. Results 2.1.

In vitro, Ku70−/− mouse fibroblasts displayed an increased rate of sister chromatid exchange and a high frequency of spontaneous neoplastic transformation. In vivo, Ku70−/− mice, known to be defective in B but not T lymphocyte maturation, developed thymic Complex: Ku70:Ku80 complex Macromolecular complex annotations are imported from the Complex Portal.These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background. In vitro, Ku70 -/- mouse fibroblasts displayed an increased rate of sister chromatid exchange and a high frequency of spontaneous neoplastic transformation. In vivo, Ku70 -/- mice, known to be defective in B- but not T-lymphocyte maturation, developed thymic and disseminated T-cell lymphomas at a mean age of 6 months with CD4+/CD8+ tumor cells. Ku70 (also known as XRCC6) is a eukaryotic protein that is involved in the repair of DNA double-strand breaks by non-homologous end-joining [ 1, 2 ] Ku is a heterodimer of approximately 70kDa and 80kDa subunits The Ku70/Ku80 heterodimer has ATP-dependent DNA helicase activity and functions as the DNA-binding regulatory component of DNA-dependent protein kinase (DNA-PK) (6-8). The assembly of the DNA-PK complex at DNA ends is required for nonhomologous end-joining (NHEJ), one mechanism involved in double-stranded DNA break repair and V(D)J recombination (8). KU70 antibody Rabbit Polyclonal from Proteintech validated in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC),Enzyme-linked Immunosorbent Assay (ELISA) applications.
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2013 Jul 29. This plasmid is available through Addgene. Se hela listan på academic.oup.com of the Ku70/80 heterodimer in vitro.

Securin 2021-02-22 · Acetylation modulates DNA-mediated Ku70-dependent IFN-λ1 induction. Ku80 colocalizes with Ku70 during Ku70 translocation from the nucleus to the cytoplasm, but not directly involving in Ku70's cytosolic DNA activity.
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Diseases associated with XRCC6 include Lupus Erythematosus and Nijmegen Breakage Syndrome.Among its related pathways are Cytosolic sensors of pathogen-associated DNA and DNA damage_NHEJ mechanisms of DSBs repair.Gene Ontology (GO) annotations related to this gene include protein C-terminus binding. ku70 tert : gl1; ku70; tert: images; None available : phenotypes ; Surovtseva YV, Shakirov EV, Vespa L, Osbun N, Song X, Shippen DE(2007) In contrast to the ku70 mutant, which undergo telomerase-dependent expansion to more than twice the normal length in a single generation, the ku70 tert double mutant displays accelerated telomere shortening and a precocious onset of genome stability. Plasmid pEGFP-C1-FLAG-Ku70 from Dr. Steve Jackson's lab contains the insert Ku70 and is published in J Cell Biol.

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Ku70 and Ku80 form a pseudosymmetrical heterodimer with a preformed ring (also called DNA aperture) responsible for sequence‐independent DNA binding. Invitrogen Anti-Ku70/Ku80 Monoclonal (162), Catalog # MA1-21818. Tested in Immunocytochemistry (ICC/IF), Immunohistochemistry (Paraffin) (IHC (P)) and Dec 5, 2006 Accumulation of XRCC4/ligase IV on DSBs depended on the presence of Ku70/ 80, but not DNA-PKCS. We detected a direct interaction between Feb 27, 2014 The KU70-deficient mutant generated herein was effective in improving gene deletion frequency and allowed shorter homology sequences to Monoclonal Antibody for studying Ku70 in the Cell Cycle / Checkpoint research area. Jul 24, 2015 ARABIDOPSIS THALIANA KU70 HOMOLOG, ATKU70, KU70, KU70 Ku80 and ku70 form the heterodimer complex Ku, required for proper May 1, 2013 The core NHEJ machinery includes the end-binding heterodimeric proteins Ku70 /Ku80, the DNA-PKcs protein kinase, and the complex Jun 20, 2012 The Ku heterodimer complex consists of Ku70 and Ku80 and is a very versatile protein that regulates a wide range of important and diverse Ku70 Antibody (E-5) is a monoclonal anti-KU70 antibody that detects m, r, and h Ku70 by WB, IP, IF, IHC(P) and ELISA.

This plasmid is available through Addgene. Invitrogen Anti-Ku70 Polyclonal, Catalog # PA5-27538. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF), Immunohistochemistry (Paraffin) (IHC (P)), Immunoprecipitation (IP) and Proximity Ligation Assay (PLA) (PLA) applications. This antibody reacts with Human samples.

In vitro, Ku70 -/- mouse fibroblasts displayed an increased rate of sister chromatid exchange and a high frequency of spontaneous neoplastic transformation. In vivo, Ku70 -/- mice, known to be defective in B- but not T-lymphocyte maturation, developed thymic and disseminated T-cell lymphomas at a mean age of 6 months with CD4+/CD8+ tumor cells. Rabbit anti Ku70 antibody recognizes human Ku70, also known as XRCC6, G22P1 or X-ray repair cross-complementing protein 6.